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Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples
品質等級
物種活性
human
參數
50 μL sample volume (4hr assay)
assay range
accuracy: 77-102 (plasma)%
linearity: 105 (plasma)%
inter-assay cv: <4.7%
intra-assay cv: <4.0%
standard curve range: 0.82-200 pg/mL
accuracy: 70-105 (serum)%
linearity: 94-106 (serum)%
技術
ELISA: suitable
輸入
sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum
UniProt登錄號
應用
research use
檢測方法
colorimetric (450nm/590nm)
基因資訊
human ... CXCL8(3576)
一般說明
IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.
應用
This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.
Analytes available:
Research Category:
Analytes available:
- IL-8
Research Category:
- Cytokines
- Inflammation & Immunology
- Immune Response
- Metabolic Disease
分析報告
3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.
法律資訊
CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
For research use only. Not for use in diagnostic procedures.
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