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Merck
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Key Documents

CYT306

Sigma-Aldrich

Rat/Human Brain Derived Neurotrophic Factor ELISA Kit

measures and quantifies BDNF levels in serum, plasma, cell culture supernatent or tissue homogenate

别名:

BDNF, Brain Derived Neurotrophic Factor

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

product name

ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA, This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.

品質等級

物種活性

human, rat

製造商/商標名

ChemiKine
Chemicon®

技術

ELISA: suitable

UniProt登錄號

應用

research use

檢測方法

colorimetric

運輸包裝

wet ice

基因資訊

human ... BDNF(627)

一般說明

ChemiKine Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

Test Principle:

With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.

Application:

ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.

應用

Research Category
Neuroscience
This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.
Used to detect/quantify: BDNF

成分

ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.

Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.

Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).

BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized).

Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial.

Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin.

TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3′,5,5′-tetramethylbenzidine in a proprietary buffer with enhancer.

Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution.

儲存和穩定性

Precautions:

· Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit′s performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

For research use only. Not for use in diagnostic procedures.

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - Skin Sens. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials


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