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Key Documents

EZCL-31K

Millipore

Canine Leptin ELISA Kit

measures Leptin in 20 μL serum, plasma or cell culture supernatent

别名:

Canine Leptin Kit, Leptin ELISA

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

product name

Canine Leptin ELISA,

物種活性

canine

品質等級

包裝

kit of 1 × 96 wells

參數

20 μL sample volume (4hr assay)

技術

ELISA: suitable

輸入

sample type serum
sample type plasma (K2 EDTA)

應用

research use

assay range

inter-assay cv: 6-7%
intra-assay cv: 2-6%
sensitivity: 0.21 ng/mL
standard curve range: 0.78-50  ng/mL
accuracy: 100-106%

檢測方法

colorimetric (450nm/590nm)

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

Leptin, a 16 kD protein and a product of the obesity (ob) gene, is synthesized and secreted predominantly by adipose tissue and is, therefore, considered an adipokine. With circulating levels directly proportional to the total amount of body fat, leptin plays an important role in whole body metabolism, regulating food intake and energy balance, as well as assisting in the control of homeostasis, reproductive function, the immune system and numerous other body functions. Binding to receptors of the hypothalamus, leptin signals food satiety and reduces appetite, although evidence suggests that, in general, obese people may have unusually high circulating levels of leptin. This may indicate a resistance to leptin similar to the insensitivity to insulin associated with type 2 diabetes. Consequently, the study of leptin is essential to understanding the relationship among appetite, energy balance and obesity.

特異性

Used to detect/quantify: Leptin

應用

This Canine Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

This Is a 4-hour room temperature assay using 20 μL sample size

Research Category: Metabolism

Research Sub-Category: Obesity, Metabolic Disorders

包裝

96-well strip plate

其他說明

Contact Technical Support.

免責聲明

For research use only. Not for use in diagnostic procedures.

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