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product name
脑源性神经营养因子 人, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
生物源
human
品質等級
重組細胞
expressed in E. coli
化驗
≥95% (SDS-PAGE)
形狀
lyophilized powder
品質
endotoxin tested
分子量
~27 kDa
包裝
pkg of 10 μg
pkg of 5 μg
儲存條件
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
技術
cell culture | mammalian: suitable
雜質
≤1.00 EU/μg Endotoxin level
UniProt登錄號
儲存溫度
−20°C
一般說明
BDNF是生长因子中神经营养蛋白家族的成员，该家族还包括NGF、NT-3和NT-4。所有神经营养因子具有六个保守的半胱氨酸残基，并且在氨基酸水平上具有55％的序列同一性。BDNF已被证明可以增强体外几类神经元的存活和分化，包括神经嵴和基板衍生的感觉神经元、黑质中的多巴胺能神经元、基底前脑胆碱能神经元、海马神经元和视网膜神经节细胞.1 BDNF在外周神经节内表达，并且不限于神经元靶区域，因而提高了BDNF对神经元以及非神经元细胞具有旁分泌或甚至自分泌作用的可能性。
人重组BDNF蛋白（27kDa）的活性形式是由两个相同的119个氨基酸亚基形成的二聚体，它们通过强疏水相互作用结合在一起
人重组BDNF蛋白（27kDa）的活性形式是由两个相同的119个氨基酸亚基形成的二聚体，它们通过强疏水相互作用结合在一起
應用
脑源性神经营养因子（BDNF）重组人蛋白已被用作各种培养基中的补充剂。
生化／生理作用
脑源性神经营养因子（BDNF）（13.6kDa或27.2kDa二聚体）是神经营养蛋白家族的成员。 BDNF在人、小鼠和猪中具有相同的氨基酸序列，具有完全的交叉反应性。 BDNF在中枢神经系统内的神经元群体或与其直接相关的细胞的发育和维持中具有重要作用。 在体外，BDNF已报到可增强几类神经元的存活和分化，其中包括神经嵴和基板衍生的感觉神经元、黑质中的多巴胺能神经元、基底前脑胆碱能神经元、海马神经元和视网膜神经节细胞。
外觀
从经过无菌0.2 μm过滤的BSA溶液中冻干得到
分析報告
其生物活性由其刺激人神经母细胞瘤细胞SH-SY5Y增殖的能力来确定。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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