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Merck
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主要文件

CA1023

Sigma-Aldrich

Anti-Hsp90α Mouse mAb (EMD-17D7)

liquid, clone EMD-17D7, Calbiochem®

别名:

Anti-Heat Shock Protein 90α

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41
無性繁殖:
EMD-17D7, monoclonal
application:
物種活性:
human
citations:
12

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

EMD-17D7, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

human

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG1

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

Protein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.
Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.

免疫原

Hsp90α, His•Tag, Human, Recombinant (Cat. No. 385901)
Human

應用



ELISA (see comments)
Immunoblotting (1 g/ml)
Immunoprecipitation (5 g/0.7 mg protein)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Irritant (B)

外觀

In PBS.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Positive Control
H460 and HeLa cells

其他說明

Detects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Wei Li et al.
The EMBO journal, 26(5), 1221-1233 (2007-02-17)
Hypoxia is a microenvironmental stress in wounded skin, where it supports wound healing by promoting cell motility. The mechanism of the hypoxia action remained speculative. Here, we provide evidence that hypoxia promotes human dermal fibroblast (HDF) migration by inducing secretion
Sun-Ii Hwang et al.
Methods in molecular biology (Clifton, N.J.), 1002, 25-35 (2013-04-30)
Cellular localization of proteins is one of the most valuable sources of information regarding spatiotemporal biological events involved in human disease. This information is sometimes enhanced by carrying out protein isolation using a process known as subcellular fractionation. This involves
Dorota Gil et al.
Cellular signalling, 72, 109642-109642 (2020-04-20)
Malignant transformation is characterized by a phenotype "switch" from E- to N-cadherin - a major hallmark of epithelial to mesenchymal transition (EMT). The increased expression of N-cadherin is commonly followed by a growing capacity for migration as well as resistance
Mohamed Ali Jarboui et al.
PloS one, 7(11), e48702-e48702 (2012-11-21)
The trans-activator Tat protein is a viral regulatory protein essential for HIV-1 replication. Tat trafficks to the nucleoplasm and the nucleolus. The nucleolus, a highly dynamic and structured membrane-less sub-nuclear compartment, is the site of rRNA and ribosome biogenesis and

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