推荐产品
生物源
human
品質等級
化驗
≥95% (SDS-PAGE)
形狀
liquid
製造商／商標名
Chemicon®
濃度
0.25 mg/mL
技術
cell culture | mammalian: suitable
輸入
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
−70°C
基因資訊
human ... LAMB1(3912)
一般說明
AG56P contains a mixture of human laminins containing the beta1 chain, principally laminin 1, 2, 3, 6, 8 & 10. This preparation is immunologically and biologically identical to intact human laminin. AG56P is suitable for use in ELISA, production of antiserum, cell adhesion and attachment assays, and neurite-stimulation assays. This pepsinized laminin preparation runs as twin bands migrating at approximately 160kDa and 130kDa by SDS-PAGE under reducing conditions.
Preparation: Chemicon′s purified human laminin is prepared from freshly frozen human placenta tissue that is homogenized in PBS with a blender and the mixture centrifuged. The pellet is then washed with 0.5M acetic acid, collected and subjected to and extended Pepsin A digestion. The pepsin digest is neutralized, centrifuged to clear debris, and applied to an antibody column containing mouse anti-human laminin monoclonal 4E10 monoclonal antibody {MAB1921}. The solubilized laminin solution is passed over the column, and utlimately the laminin is released via acidification with KSCN. Then the material is dialyzed against PBS. The antibody 4E10 is specific for laminin beta1 chain. This chain is found in Laminin′s 1,2,3,6,8,10 under the native conditions used to purify the material.
Preparation: Chemicon′s purified human laminin is prepared from freshly frozen human placenta tissue that is homogenized in PBS with a blender and the mixture centrifuged. The pellet is then washed with 0.5M acetic acid, collected and subjected to and extended Pepsin A digestion. The pepsin digest is neutralized, centrifuged to clear debris, and applied to an antibody column containing mouse anti-human laminin monoclonal 4E10 monoclonal antibody {MAB1921}. The solubilized laminin solution is passed over the column, and utlimately the laminin is released via acidification with KSCN. Then the material is dialyzed against PBS. The antibody 4E10 is specific for laminin beta1 chain. This chain is found in Laminin′s 1,2,3,6,8,10 under the native conditions used to purify the material.
Product Source: Human placenta, tested negative for hepatitis B virus, hepatitis C virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2, and Treponema pallidum. Handle as if potentially infectious.
品質
Each lot is analyzed on SDS-PAGE for band integrity and expected sizes prior to packaging and release
外觀
Purified laminin protein in liquid in Tris Buffer Saline with 0.01% sodium azide.
準備報告
Purified from a mild pepsin digestion followed by affinity chromatography on monoclonal (4E10) anti-human laminin-Sepharose.
儲存和穩定性
Store at -20°C or -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a table-top centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a table-top centrifuge to dislodge any liquid in the container′s cap.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Domenico Vitolo et al.
The American journal of pathology, 168(3), 991-1003 (2006-03-02)
Capillaries expressing the laminin alpha2 chain in basement membranes may be considered early developing vessels in normal and neoplastic human tissues. Therefore, we investigated whether up-regulation of this extracellular matrix protein favors transendothelial migration of neoplastic cells and then metastasis.
Timothy D Culp et al.
Journal of virology, 80(18), 8940-8950 (2006-08-31)
Human papillomaviruses (HPVs) replicate only in the terminally differentiating epithelium of the skin and mucosa. While infection of basal keratinocytes is considered a requirement for permissive infection, it remains unclear whether virions can specifically target basal cells for adsorption and
Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
Engvall, E, et al.
The Journal of cell biology, 103, 2457-2465 (1986)
Sebastian Wurster et al.
Cell reports, 34(12), 108896-108896 (2021-03-25)
Severe and often fatal opportunistic fungal infections arise frequently following mucosal damage caused by trauma or cytotoxic chemotherapy. Interaction of fungal pathogens with epithelial cells that comprise mucosae is a key early event associated with invasion, and, therefore, enhancing epithelial
商品
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
相关内容
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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