ABS2053

Anti-Glutaredoxin-2 (Grx2)

from rabbit, purified by affinity chromatography

• 别名: Glutaredoxin-2, mitochondrial
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: baboon (based on 100% sequence homology), monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NP_001230328
• UniProt登錄號: Q9NS18
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GLRX2(51022)

说明

一般說明

Glutaredoxin-2, mitochondrial (UniProt: Q9NS18) is encoded by the GLRX2 (also known as GRX2, CGI-133) gene (Gene ID: 51022) in human. Glutaredoxin-2 is a glutathione-dependent oxidoreductase that is widely expressed and facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Its active form is a monomer and it can dimerize to an inactive form where homodimers are linked by one 2Fe-2S cluster. The 2Fe-2S may serve as a redox sensor and the presence of one-electron oxidants or reductants can lead to the loss of the 2Fe-2S cluster and subsequent monomerization and activation of the enzyme. Glutaredoxin-2 acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. It can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Glutaredoxin-2 is known to efficiently catalyze both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression of glutaredoxin-2 decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.

特異性

This rabbit polyclonal antibody detects mitochondrial isoform of glutatedoxin-2. It targets an epitope with in 16 amino acids from the C-terminal half.

免疫原

Epitope: unknown

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Glutaredoxin-2 .

應用

Anti-Glutaredoxin-2 (Grx2), Cat. No. ABS2053, is a rabbit polyclonal antibody that detects Glutaredoxin-2, mitochondrial, and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glutaredoxin-2 (Grx2) in human cerebellum and human tonsil tissue.

Research Category
Signaling

品質

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glutaredoxin-2 (Grx2) in 10 µg of A549 cell lysate.

標靶描述

~17 kDa observed; 18.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable