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生物源
sheep
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human
技術
ChIP: suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... POLR2M(81488)
一般說明
Gdown1 (DNA-directed RNA polymerase II subunit GRINL1A; DNA-directed RNA polymerase II subunit M; glutamate receptor-like protein 1A) is a nuclear protein belonging to the GRINL1 family. It binds to the RNA Polymerase II (G) molecule (RNAPII (G)) and prevents its interaction with transactivating factors, thereby suppressing RNAPII (G)-mediated transcriptional events. Gdown1 also indirectly suppresses RNAPII (G)-transcription by blocking the binding of the elongation-enhancing factor TFIIF to the elongation complex. Gdown1 also prevents TFIIF-mediated assembly of the preinitiation complex required for recruiting RNAPII to the promoter region, by binding competitively to Pol II at the RPB1 and RPB5 subunits recognized by TFIIF. Gdown1 may also inhibit the termination factor TTF2. The effects of Gdown1 are blocked by the P-TEFb. Overall, Gdown1 regulates the transcriptional activity of RNAPII (G) at multiple promoter regions by various mechanisms. Gdown1 has been detected in brain, testis, prostate, lung, small intestine, heart, kidney, and skeletal muscle.
免疫原
Histidine-tagged recombinant protein corresponding to human Gdown1.
應用
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)
Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)
Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Use Anti-Gdown1 Antibody (Sheep Polyclonal Antibody) validated in WB, ChIP to detect Gdown1 also known as Myocardial zonula adherens protein, GRINL1A upstream protein, Gup.
品質
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate.
標靶描述
~50 kDa observed. Uniprot describes 11 isoforms ranging between 44 and 64 kDa
外觀
Affinity purified
Purified sheep polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
HeLa cell lysate
HeLa cell lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Bo Cheng et al.
Molecular cell, 45(1), 38-50 (2012-01-17)
Most human genes are loaded with promoter-proximally paused RNA polymerase II (Pol II) molecules that are poised for release into productive elongation by P-TEFb. We present evidence that Gdown1, the product of the POLR2M gene that renders Pol II responsive
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