540221

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

• 别名: PNP
• 分類程式碼代碼: 12352202
• NACRES: NA.42

属性

• 重組細胞: expressed in E. coli
• 品質等級: 100
• 形狀: liquid
• 比活性: ≥25 units/mg protein
• 製造商／商標名: Calbiochem^®
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

外觀

In 50 mM Tris-HCl, pH 7.6.

分析報告

Single band by SDS-PAGE

其他說明

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable