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重組細胞
expressed in E. coli
形狀
powder
比活性
≥2.7 units/mg solid
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
吡喃糖氧化酶(P20),是由共价结合的黄素腺嘌呤二核苷酸组成的同型四聚体蛋白质,主要存在于担子菌类木材腐朽菌中。
應用
吡喃糖氧化酶(来源于云芝)已用于D-葡萄糖(DG)的酶法氧化。它还可作为氧清除系统(OSS)组分以延长荧光团寿命。
生化/生理作用
吡喃糖氧化酶(P2O)催化吡喃醛糖C-2位氧化成为相应的2-酮醛糖。吡喃糖氧化酶的体内底物据认为是D-葡萄糖、D-半乳糖和D-木糖。 它们可分别被氧化成为2-酮基-D-葡萄糖(D-阿拉伯-己-2-酮糖,2-脱氢-D-葡萄糖)、2-酮基-D-半乳糖(D-来苏型-己-2-酮糖,2-脱水-D-半乳糖)和2-酮基-D-木糖(D-苏型-戊-2-酮糖,2-脱水-D-木糖)。吡喃糖氧化酶对L-山梨糖、D-葡萄糖酸-1,5-内酯和D-阿洛糖等糖类有显著活性。 当吡喃糖氧化酶催化吡喃醛糖的氧化时,电子转移到分子氧上,从而形成过氧化氢。
吡喃糖氧化酶(P2O)在临床化学中可用于测定1,5-脱水-d-葡萄糖醇标志物,用于糖尿病患者的血糖控制。
單位定義
一个单位在 37°C,pH7.0 下每分钟产生 1.0μ 摩尔过氧化氢。
其他說明
含谷氨酸
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
The influence of temperature on bioconversion of D-glucose into 2-keto-D-glucose by Pyranose oxidase
UPB Scientific Bulletin, Series B: Chemistry and Materials Science, 2 (2014)
Microbial cell factories, 9, 14-14 (2010-03-11)
The heterologous production of the industrially relevant fungal enzyme pyranose 2-oxidase in the prokaryotic host E. coli was investigated using 3 different expression systems, i.e. the well-studied T7 RNA polymerase based pET21d+, the L-arabinose inducible pBAD and the pCOLD system.
Nature communications, 8(1), 1830-1830 (2017-12-01)
The neural crest is an embryonic population of multipotent stem cells that form numerous defining features of vertebrates. Due to lack of reliable techniques to perform transcriptional profiling in intact tissues, it remains controversial whether the neural crest is a
The Journal of biological chemistry, 285(13), 9697-9705 (2010-01-22)
Pyranose 2-oxidase (P2O) catalyzes the oxidation by O(2) of d-glucose and several aldopyranoses to yield the 2-ketoaldoses and H(2)O(2). Based on crystal structures, in one rotamer conformation, the threonine hydroxyl of Thr(169) forms H-bonds to the flavin-N5/O4 locus, whereas, in
Journal of molecular biology, 402(3), 578-594 (2010-08-17)
Flavoenzymes perform a wide range of redox reactions in nature, and a subclass of flavoenzymes carry covalently bound cofactor. The enzyme-flavin bond helps to increase the flavin's redox potential to facilitate substrate oxidation in several oxidases. The formation of the
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