推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
PY20, monoclonal
形狀
liquid
包含
0.1% Proclin as preservative
物種活性（以同源性預測）
all
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG2b
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
phosphorylation (pTyr)
基因資訊
human ... PID1(55022)
一般說明
Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.
免疫原
KLH conjugated to phosphotyrosine, glycine, and alanine
應用
ELISA (1:2000)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外觀
In PBS, 50% glycerol, 1% BSA, pH 7.4.
其他說明
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Andrew J Garton et al.
Cancer research, 66(2), 1015-1024 (2006-01-21)
OSI-930 is a novel inhibitor of the receptor tyrosine kinases Kit and kinase insert domain receptor (KDR), which is currently being evaluated in clinical studies. OSI-930 selectively inhibits Kit and KDR with similar potency in intact cells and also inhibits
Justin M Scheer et al.
PloS one, 7(12), e51817-e51817 (2013-01-04)
The structure of the Fab region of antibodies is critical to their function. By introducing single cysteine substitutions into various positions of the heavy and light chains of the Fab region of trastuzumab, a potent antagonist of HER2, and using
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