推荐产品
产品名称
磷酸酶抑制剂混合物套装V，50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
形狀
liquid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
protect from light
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
一种由四种磷酸酶抑制剂组成的混合物，可同时抑制丝氨酸/苏氨酸和蛋白酪氨酸磷酸酶。可以1 ml或一组5个1 ml小瓶的形式提供。
應用
磷酸酶抑制剂混合物套装V(50X)的应用实例：
- 加入裂解缓冲液以分离代谢活化(metabolically activated one)骨髓巨噬细胞(BMDM)用于蛋白质免疫印迹
- 加入放射性免疫沉淀(RIPA)改良缓冲液以孵育人脐静脉内皮细胞(HUVEC)培养物用于蛋白质免疫印迹
- 加入裂解缓冲液以裂解HUVEC培养物用于免疫沉淀
- 加入洗涤缓冲液以洗涤蛋白复合物
- 加入4-羟乙基哌嗪乙磺酸(HEPES)缓冲液以均质化J774细胞，用于评估脂多糖(LPS)孵育对细胞质磷脂酶A2(cPLA2)活性的影响
生化／生理作用
蛋白磷酸化和去磷酸化在信号转导中起重要作用，调控细胞分裂、增殖和凋亡等各种细胞过程。蛋白质激酶会给特定位点的丝氨酸、苏氨酸或酪氨酸残基加上磷酸基团，改变蛋白的构象及与配体相互作用的能力。接着蛋白质磷酸酶去除磷酸基团，使蛋白质恢复原本的去磷酸化状态。从细胞和组织裂解物中提取磷酸化蛋白时，保持它们的磷酸化状态至关重要。
特點和優勢
- 方便：即用型制剂，使用便捷。
- 稳定：高品质制剂，保证可靠、可重复性能。
- 多用：该混合物靶向不同类别的磷酸酶，在实验中发挥多功能作用。
成分
每小瓶含1 mL包含下列组分的水溶液：
- 250 mM氟化钠
- 50 mM ß-磷酸甘油（货号35675）
- 50 mM十水焦磷酸钠
- 50 mM原钒酸钠
警告
毒性：有毒（F）
外觀
在H₂O中。
重構
初次解冻后，分装并冷冻（-20°C）以进行长期储存。临用前按1：50稀释。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Umberto Tosi et al.
Science advances, 6(30), eabb4105-eabb4105 (2020-08-25)
Efforts at altering the dismal prognosis of pediatric midline gliomas focus on direct delivery strategies like convection-enhanced delivery (CED), where a cannula is implanted into tumor. Successful CED treatments require confirmation of tumor coverage, dosimetry, and longitudinal in vivo pharmacokinetic
Hongxu Sheng et al.
International journal of molecular medicine, 46(3), 1039-1050 (2020-07-25)
Although significant progress has been made in the treatment of lung cancer, it remains the leading cause of cancer‑associated mortality. Liriopesides B (LPB) is a natural product isolated from the tuber of Liriope platyphylla, whose effective substances have exhibited antitumor activity in
Sonia Sabate-Soler et al.
Glia, 70(7), 1267-1288 (2022-03-10)
The human brain is a complex, three-dimensional structure. To better recapitulate brain complexity, recent efforts have focused on the development of human-specific midbrain organoids. Human iPSC-derived midbrain organoids consist of differentiated and functional neurons, which contain active synapses, as well
Linhai Zhu et al.
International journal of molecular medicine, 48(6) (2021-10-14)
Schizandrin A (SchA) can be extracted from the vine plant Schisandra chinensis and has been reported to confer various biologically active properties. However, its potential biological effects on non‑small cell lung cancer (NSCLC) remain unknown. Therefore, the present study aims to
Fiona Bartoli et al.
The Journal of clinical investigation, 132(5) (2022-01-14)
Piezo1 forms mechanically activated nonselective cation channels that contribute to endothelial response to fluid flow. Here we reveal an important role in the control of capillary density. Conditional endothelial cell-specific deletion of Piezo1 in adult mice depressed physical performance. Muscle
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门