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Key Documents

420400

Sigma-Aldrich

Anti-KDEL Mouse mAb (10C3)

liquid, clone 10C3, Calbiochem®

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

10C3, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG2a

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

mouse ... Kdelr1(68137)

一般說明

Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Recognizes the Grp78 (BiP) and Grp94 proteins.
This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

免疫原

Rat
a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH

應用

Immunoblotting (4 µg/ml, colorimetric)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (5 µg/ml)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Highly Toxic (H)

外觀

In PBS, 50% glycerol.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells

其他說明

Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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