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一般說明
蛋白A是从金黄色葡萄球菌Cowan I菌株的细胞壁分离的42 kDa单链多肽。
特異性
对蛋白A具有特异性,与蛋白G无交叉反应性。与游离蛋白A或IgG结合蛋白A反应,不干扰蛋白A的Fc结合活性。
免疫原
来自金黄色葡萄球菌Cowan I菌株的蛋白A。
應用
小鼠抗蛋白A单克隆抗体可用于酶联免疫吸附测定(ELISA)。它还可用于蛋白质印迹和斑点印迹分析。
生化/生理作用
由于蛋白A对许多哺乳动物免疫球蛋白的Fc区具有亲和力,因此被认为是生物化学和免疫学中的通用试剂。它可用于不同的应用,例如通过亲和色谱法纯化免疫球蛋白,细胞表面研究,放射免疫测定(RIA),酶免疫测定(EIA),免疫沉淀和许多其他程序。它可以以其天然形式或与各种标记物偶联的形式用于这些方法。
外觀
小鼠中产生的单克隆抗蛋白A抗体以含15 mM叠氮化钠的腹水形式提供。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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Journal of immunological methods, 217(1-2), 143-151 (1998-10-17)
Accuracy of antigen determination in human plasma samples is often adversely affected by immune complex formation between antigens (e.g., HIV-1 p24 protein) and specific antibodies. In this study we describe an optimized method for complete immune complex dissociation (ICD) in
Comparison of protein A affinity sorbents III. Life time study
Journal of Chromatography A, 1102(1-2), 224-231 (2006)
Molecular microbiology, 78(1), 238-252 (2010-10-07)
The human pathogen Staphylococcus aureus requires cell wall anchored surface proteins to cause disease. During cell division, surface proteins with YSIRK signal peptides are secreted into the cross-wall, a layer of newly synthesized peptidoglycan between separating daughter cells. The molecular
Journal of chromatography. A, 1102(1-2), 224-231 (2005-12-06)
Protein A affinity chromatography is a popular purification method for immunoglobulins applied at various scales, ranging from micro-tube up to 1000l column format. Three novel high capacity protein A affinity chromatography media have been subjected to a lifetime study using
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