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Merck
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主要文件

14-685-M

Sigma-Aldrich

Cdk9/Cyclin T1 Protein, active, 10 µg

Active, C-terminal 6His-tagged, full length recombinant, human Cdk9 co-expressed with untagged, full-length human Cyclin T1, for use in Kinase Assays.

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.32

生物源

human

品質等級

100

重組細胞

expressed in Sf9 cells

分子量

Mw 44 kDa (cdk9)
Mw 80.79 kDa (cyclinT1)

製造商／商標名

Upstate®

技術

activity assay: suitable (kinase)

NCBI登錄號

NM_001261.2

UniProt登錄號

P50750

基因資訊

human ... CDK9(1025)

一般說明

C-terminal 6His-tagged, fulllength recombinant, human cdk9 co-expressed with untagged, full-length human cyclin T1
Product Source: expressed by baculovirus in Sf21 insect cells

應用

Research Category
Inflammation & Immunology

生化／生理作用

Protein Target: CDK9/cyclin T1
Target Sub-Family: CMGC

品質

routinely evaluated by phosphorylation of PDKtide (Catalog# 12-401)

外觀

Ni2+/NTA-agarose

儲存和穩定性

stable for 6 months at -70°C from date of shipment

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cyclins that don't cycle--cyclin T/cyclin-dependent kinase-9 determines cardiac muscle cell size.
Sano, Motoaki and Schneider, Michael D
Cell Cycle, 2, 99-104 (2003)
Cellular control of gene expression by T-type cyclin/CDK9 complexes.
Garriga, Judit and Gra?a, Xavier
Gene, 337, 15-23 (2004)
Li-Ying Liou et al.
The international journal of biochemistry & cell biology, 36(9), 1767-1775 (2004-06-09)
The macrophage is an important cell type in the pathophysiology of human immunodeficiency virus type 1 (HIV-1) infection. Macrophages both support viral replication and are capable of attracting and activating lymphocytes, thus rendering CD4+ T lymphocytes highly permissive for infection.
Tinggui Yin et al.
Molecular cancer therapeutics, 13(6), 1442-1456 (2014-04-02)
DNA-dependent RNA polymerase II (RNAP II) largest subunit RPB1 C-terminal domain (CTD) kinases, including CDK9, are serine/threonine kinases known to regulate transcriptional initiation and elongation by phosphorylating Ser 2, 5, and 7 residues on CTD. Given the reported dysregulation of

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