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Merck
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主要文件

05-623-AF647

Sigma-Aldrich

Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor 647 Conjugate

clone CTD4H8, from mouse, ALEXA FLUOR 647

别名:

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
共軛:
ALEXA FLUOR 647
application:
ICC
無性繁殖:
CTD4H8, monoclonal
物種活性:
mouse, human
citations:
技術:
immunocytochemistry: suitable

生物源

mouse

品質等級

共軛

ALEXA FLUOR 647

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

CTD4H8, monoclonal

物種活性

mouse, human

物種活性(以同源性預測)

rat (based on 100% sequence homology), yeast (based on 100% sequence homology)

技術

immunocytochemistry: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)

一般說明

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

特異性

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

免疫原

Epitope: carboxyl-terminal domain of RNA polymerase II
KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

應用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

品質

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in HeLa cells.

標靶描述

~210-220 kDa observed

外觀

Protein G Purified
Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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