05-1951-S

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1, Trial Size

clone 18E9.1, from mouse

• 别名: H3K27me3, Histone H3 (trimethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 18E9.1, monoclonal
• 物種活性: human, mouse
• 技術: dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_002098
• UniProt登錄號: P84243
• 運輸包裝: wet ice
• 目標翻譯後修改: trimethylation (Lys27)
• 基因資訊: human ... H3C1(8350) ; mouse ... H3C1(360198)

说明

一般說明

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特異性

This antibody recognizes Histone H3 trimethylated at Lys27.

免疫原

Epitope: Trimethylated Lys27

Linear peptide corresponding to human Histone H3 trimethylated at Lys27.

應用

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1, Trial Size / Pack is a mouse monoclonal antibody for detection of trimethyl Histone H3 (Lys27) also known as H3K27me3, Histone H3 (tri methyl K27) & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of modified and nonmodified peptides.

Western Blot (Snap i.d.) Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of HeLa acid extract.

Immunocytochemistry Analysis: A 1:125 dilution from a representative lot detected Histone H3 in A431 and NIH/3T3 cells.

Dot Blot Analysis: Histone peptides with various modifications (see table) were transferred to
PVDF membrane and probed with Anti-Trimethyl Histone H3 (Lys4) (1:100 dilution). Proteins were visualized using a Donkey Anti-rabbit IgG conjugated to HRP and a chemiluminescence detection systems.

品質

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: A 1:50,000 dilution of this antibody detected Histone H3 in 10 µg of HeLa acid extract.

標靶描述

~17 kDa observed

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa acid extract

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable