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Merck

767964

Sigma-Aldrich

细胞游离氨基酸混合物 - 13C,15N

5-100 mM in water, 98 atom % 15N, 98 atom % 13C

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About This Item

分類程式碼代碼:
12352209
NACRES:
NA.12

同位素純度

98 atom % 13C
98 atom % 15N

形狀

solid

濃度

5-100 mM in water

技術

bio NMR: suitable
protein expression: suitable

運輸包裝

dry ice

儲存溫度

−20°C

應用

该产品适合作为无细胞蛋白质合成的稳定同位素标记氨基酸的来源。对于质谱应用,考虑产品909653,用于质谱的稳定同位素标记氨基酸混合物。

由于酪氨酸的浓度相对较高,产品可能会出现浑浊。一旦稀释使用,溶液应澄清。

包裝

所有20种氨基酸的1 mL水溶液。
该产品可以大包装形式供货,也可按需进行包装。 如需关于价格、供货形式及包装的信息,请联系稳定同位素客户服务

分析報告

氨基酸浓度因批次而异。请参见特定批次检验报告。

近似浓度(mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20

需要但未提供

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Preparation of amino acid mixtures for cell-free expression systems
Caschera, F., Noireaux, V
Biotechniques, 58, 40-43 (2015)
Estelle Couradeau et al.
Nature communications, 10(1), 2770-2770 (2019-06-27)
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare
Zhenzhen Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 72-81 (2019-06-10)
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 proteinogenic l-amino acids (AAs) in a small volume (5 μL) of mouse plasma. Chromatographic separation was achieved on an Intrada Amino Acid column within 13 min via
Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all
Sara Calhoun et al.
Communications biology, 4(1), 333-333 (2021-03-14)
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus

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