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Merck

SAB4200720

Sigma-Aldrich

Anti-β-Catenin antibody, Mouse monoclonal

clone 15B8, purified from hybridoma cell culture

Sinónimos:

Anti-CTNNB1, Anti-armadillo

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

15B8, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~94 kDa

reactividad de especies

chicken, mouse, human, rat, monkey, hamster, bovine, canine

concentración

~1.0 mg/mL

técnicas

immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract
immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Descripción general

Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily.

Inmunógeno

recombinant chicken ß-Catenin

Aplicación

Anti-β-Catenin antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Acciones bioquímicas o fisiológicas

β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Beyond canonical: the Wnt and beta-catenin story
Moon RT and Gough NR
Science Signaling, 9(422), eg5-eg5 (2016)
Kiyomi Tsuji-Tamura et al.
FEBS letters, 596(13), 1686-1699 (2022-04-02)
Blood vessels normally remain stable over the long term. However, in atherosclerosis, vascular cells leave the quiescent state and enter an activated state. Here, we investigated the factors that trigger the breakage of the quiescent state by screening growth factors
Thomas Andl et al.
Development (Cambridge, England), 131(10), 2257-2268 (2004-04-23)
Bone morphogenetic protein (BMP) signaling is thought to perform multiple functions in the regulation of skin appendage morphogenesis and the postnatal growth of hair follicles. However, definitive genetic evidence for these roles has been lacking. Here, we show that Cre-mediated
E Sadot et al.
Oncogene, 19(16), 1992-2001 (2000-05-10)
Beta-catenin and plakoglobin are closely related armadillo family proteins with shared and distinct properties; Both are associated with cadherins in actin-containing adherens junctions. Plakoglobin is also found in desmosomes where it anchors intermediate filaments to the desmosomal plaques. Beta-catenin, on

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