S0389

Sacarosa

for molecular biology, ≥99.5% (GC)

• Sinónimos: α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopiranosil β-D-fructofuranósido, ββ-D-Fructofuranosil-α-D-glucopiranósido, D(+)-Sacarosa, Azúcar
• Fórmula empírica (notación de Hill): C₁₂H₂₂O₁₁
• Número de CAS: 57-50-1
• Peso molecular: 342.30
• Beilstein: 90825
• Número CE: 200-334-9
• Número MDL: MFCD00006626
• Código UNSPSC: 12352201
• ID de la sustancia en PubChem: 24899423
• NACRES: NA.31

Propiedades

• origen biológico: sugar cane
• Nivel de calidad: 300
• grado: Molecular Biology; for molecular biology
• Ensayo: ≥99.5% (GC)
• Formulario: crystals
• técnicas: HPLC: suitable; cryopreservation: suitable; electron microscopy: suitable; enzyme immunoassay: suitable; gas chromatography (GC): suitable; immunohistochemistry: suitable
• impurezas: ≤0.1% free glucose
• color: white
• intervalo de pH útil: 5.5-7 (25 °C, 342 g/L)
• mp: 185-187 °C (lit.)
• solubilidad: H₂O: soluble 50 g + 50 mL
• trazas de catión: heavy metals (as Pb): ≤5 ppm
• Absorción: ≤0.15 at 280 at 50%; ≤0.20 at 260 at 50%
• idoneidad: suitable for molecular biology
• aplicaciones: agriculture; agriculture; life science and biopharma; metabolomics; sample preservation
• actividad extraña: DNase, RNase, protease, none detected
• temp. de almacenamiento: room temp
• cadena SMILES: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• Clave InChI: CZMRCDWAGMRECN-UGDNZRGBSA-N

Descripción

Descripción general

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Aplicación

Sucrose has been used:

• to perform cryoprotection for kidney tissues
• as a component in the solid germination medium to germinate pollen grains
• in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
• in the cryoprotection of fixed brain tissues for immunohistochemistry

Acciones bioquímicas o fisiológicas

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Características y beneficios

• Ideal for Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Tested for DNase, RNase, and proteases
• Molecular biology grade sucrose, sourced from sugar cane

Otras notas

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For additional information on our range of Biochemicals, please complete this form.

To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)