S0266

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

• Sinónimos: 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
• Fórmula lineal: NH₂(CH₂)₃NH(CH₂)₄NH₂
• Número de CAS: 124-20-9
• Peso molecular: 145.25
• Beilstein: 1698591
• Número CE: 204-689-0
• Número MDL: MFCD00008229
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 24899434
• NACRES: NA.52

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Análisis: ≥98%
• formulario: solid or liquid
• mol peso: 145.25 (AMU)
• técnicas: cell culture | mammalian: suitable
• índice de refracción: n20/D 1.479 (lit.)
• densidad: 0.925 g/mL at 25 °C (lit.)
• actividad extraña: DNase, RNase, and protease, none detected
• temp. de almacenamiento: room temp
• cadena SMILES: NCCCCNCCCN
• InChI: 1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
• Clave InChI: ATHGHQPFGPMSJY-UHFFFAOYSA-N
• Información sobre el gen: rat ... Ppm1a(24666)

Descripción

Descripción general

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Aplicación

Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Acciones bioquímicas o fisiológicas

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Precaución

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Danger
• Frases de peligro: H314
• Consejos de prudencia: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Clasificaciones de peligro: Eye Dam. 1 - Skin Corr. 1B
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): 233.6 °F - closed cup
• Punto de inflamabilidad (°C): 112 °C - closed cup
• Equipo de protección personal: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter