R0525

RANK Ligand from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinónimos: Osteoclast differentiation factor (ODF), Osteoprotegerin ligand (OPGL), Receptor activator of NF-κB Ligand (RANKL), TNF-related activation−induced cytokines (TRANCE)
• Número MDL: MFCD01867142
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Análisis: ≥98% (SDS-PAGE)
• formulario: lyophilized powder
• potencia: 10.0-25.0 ng/mL ED₅₀
• envase: pkg of 10 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 ng/mg; <1 EU/μg
• color: white
• Nº de acceso UniProt: O35235
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... Tnfsf11(21943)

Descripción

Descripción general

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.

Aplicación

RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.

The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction.

Acciones bioquímicas o fisiológicas

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG). Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.

Forma física

Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.

Nota de análisis

The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED₅₀ for this effect is ≤10 ng/ml.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)