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M5017

Sigma-Aldrich

Medium 199

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

Nombre del producto

Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Cantidad

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitución

Supplement with 2.2 g/L sodium bicarbonate.

suplemento

Referencia del producto
Descripción
Precios

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLBX3254
SLBW8761
SLBW8690
SLBT6440
SLBT5523

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Artículos

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Contenido relacionado

Medium 199 Formulation

Medium 199 Formulation

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