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Merck

N6635

Sigma-Aldrich

Nutrient Mixture F-10 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Ham’s F-10

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Aplicación

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit
  • to culture satellite cells from fish white muscle of trout
  • for culturing neonatal porcine islets (NPIs)

Cantidad

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitución

Supplement with 1.2 g/L sodium bicarbonate.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Z Bravo et al.
Reproduction in domestic animals = Zuchthygiene, 53(3), 629-635 (2018-02-24)
The follicular fluid exerts an effect on the sperm capacitation of several species; however, these effects vary according to species, both in the sperm motility and in the subsequent acrosome reaction. In this study, the effect of alpaca follicular fluid
Ensieh Sajadi et al.
Journal of cellular biochemistry, 120(10), 17312-17325 (2019-05-22)
The current study was conducted to assess the relationship between testicular cells in spermatogenesis, through which the production of healthy and mature sperm is essential. However, it seems necessary to obtain more information about the three-dimensional pattern of the testis
Katsusuke Hata et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(2), 498-510 (2017-11-28)
The neural cell adhesion molecule (NCAM) is expressed both presynaptically and postsynaptically during neuromuscular junction formation. Genetic deletion in mice of all three isoforms (180, 140, and 120 kDa), or just the 180 isoform, suggested that different isoforms played distinct
Lu Yue et al.
STAR protocols, 1(3), 100128-100128 (2020-12-31)
Quiescent muscle stem cells, also called satellite cells (SCs), are essential for muscle regeneration. However, quiescent SCs are quickly activated during fluorescence-activated cell sorting (FACS) isolation. Here, we present an optimized protocol to isolate quiescent muscle stem cells from fixative-perfused
Cara E Ellis et al.
BioResearch open access, 4(1), 188-197 (2015-08-27)
Since the development of the Edmonton protocol, islet transplantation is increasingly encouraging as a treatment for type 1 diabetes. Strategies to ameliorate problems with the intraportal site include macroencapsulating the islets in diverse biomaterials. Characterization of these biomaterials is important

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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