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M0393

Sigma-Aldrich

Medium 199

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

M199 Medium, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

product name

Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Aplicación

Medium 199 has been used:
  • to test and determine the pH sensitivity of the sensors in a complex environment
  • as a culture medium during oocyte harvesting
  • as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
  • to culture visceral adipose tissue explants

Cantidad

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitución

Supplement with 0.35 g/L sodium bicarbonate.

suplemento

Referencia del producto
Descripción
Precios

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Song Wang et al.
Frontiers in physiology, 8, 1004-1004 (2017-12-23)
P311 was identified to markedly promote cutaneous wound healing by our group. Angiogenesis plays a key role in wound healing. In this study, we sought to define the role of P311 in skin wound angiogenesis. It was noted that P311
Thomas H C Sit et al.
Nature, 586(7831), 776-778 (2020-05-15)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first detected in Wuhan in December 2019 and caused coronavirus disease 2019 (COVID-19)1,2. In 2003, the closely related SARS-CoV had been detected in domestic cats and a dog3. However, little is known about the
Gonçalo Fernandes et al.
Human reproduction (Oxford, England), 31(2), 287-297 (2015-12-20)
Could drugs targeting ATP-sensitive K(+) (K(ATP)) channels prevent any spontaneous increase in intracellular Ca(2+) that may occur in human metaphase II (MII) oocytes under in vitro conditions? Pinacidil, a K(ATP) channel opener, and glibenclamide, a K(ATP) channel blocker, prevent a
Alberto Stracuzzi et al.
Biomechanics and modeling in mechanobiology, 20(2), 751-765 (2021-02-04)
Probing mechanical properties of cells has been identified as a means to infer information on their current state, e.g. with respect to diseases or differentiation. Oocytes have gained particular interest, since mechanical parameters are considered potential indicators of the success
Johannes Dittmann et al.
Journal of the mechanical behavior of biomedical materials, 77, 764-775 (2017-08-12)
The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical
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Artículos

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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