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D5523

Sigma-Aldrich

Medio de Eagle modificado de Dulbecco, poca glucosa

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

DME, DMEM

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About This Item

Número MDL:
MFCD00217342
Código UNSPSC:
12352207
NACRES:
NA.75

Nivel de calidad

500

formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Aplicación

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Cantidad

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitución

Supplement with 3.7 g/L sodium bicarbonate.

suplemento

Referencia del producto
Descripción
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Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317,H319

Clasificaciones de peligro

Eye Irrit. 2 - Skin Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Daniela Steffens et al.
Journal of biomaterials applications, 32(5), 663-676 (2017-10-11)
Laminin-functionalized poly-d,l-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were implanted. The analysis is comprised of the visual aspect and mean
Hsi-Hsien Hsu et al.
Journal of cellular physiology, 233(7), 5458-5467 (2017-12-17)
Oxaliplatin (OXA), is a third generation platinum drug used as first-line chemotherapy in colorectal cancer (CRC). Cancer cells acquires resistance to anti-cancer drug and develops resistance. ATP-binding cassette (ABC) drug transporter ABCG2, one of multidrug resistance (MDR) protein which can
Gayatri Jamwal et al.
Biochimica et biophysica acta, 1865(6), 920-931 (2018-04-06)
IGF1R is a ubiquitous receptor tyrosine kinase that plays critical roles in cell proliferation, growth and survival. Clinical studies have demonstrated upregulation of IGF1R mediated signaling in a number of malignancies including colon, breast, and lung cancers. Overexpression of the
Akon Higuchi et al.
Scientific reports, 5, 10217-10217 (2015-05-15)
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs
Ming-Cheng Chen et al.
Environmental toxicology, 33(5), 587-593 (2018-02-13)
Colorectal cancer (CRC) is one of the most common cancers and causes of cancer-related death. There are several first-line chemotherapeutic drugs used to treat CRC. Oxaliplatin (OXA) is an alkylating cytotoxic agent that is usually combined with other chemotherapeutic drugs
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Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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