L5390

Lectin from Erythrina cristagalli (coral tree)

lyophilized powder

• Sinónimos: Erythrina cristagalli agglutinin, ECA
• Número MDL: MFCD00163567
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• Formulario: lyophilized powder
• Nivel de calidad: 200
• potencia: <0.5 μg per mL agglutination activity
• composición: Protein, ≥85% biuret
• impurezas: salt, free
• temp. de almacenamiento: −20°C

Descripción

Acciones bioquímicas o fisiológicas

Lectin or haemagglutinin is a sugar stereospecific binding protein or glycoprotein. It is capable of reversibly agglutinating cells. It is implicated in various cellular processes such as cell agglutination, homing of leukocytes, induction of mitosis in lymphocytes, interferon c (IFN c) and cytotoxicity. It is used as a blood typing reagent in immunohaematology. Lectin is also used as a reagent in testing erythrocyte polyagglutinable states. It is considered as a potential tool for analyzing cell surface architecture and related membrane functions.

ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.

Nota de preparación

Purified by affinity chromatography.

Nota de análisis

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25 °C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)