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Merck

H5666

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Sinónimos:

GM-CSF human

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

product name

Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

origen biológico

human

Nivel de calidad

recombinante

expressed in HEK 293 cells

Análisis

≥95% (SDS-PAGE)

formulario

lyophilized powder

potencia

≤5 ng/mL EC50

calidad

endotoxin tested

mol peso

dimer 15-36 kDa (glycosylated)

envase

pkg of 5X10 μg
pkg of 10 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

técnicas

cell culture | mammalian: suitable

impurezas

≤1 EU/mg

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CSF2(1437)

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Descripción general

Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.

Aplicación

Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).

Acciones bioquímicas o fisiológicas

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Precaución

Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

Forma física

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Nota de preparación

HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

Nota de análisis

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Información legal

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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K Huebner et al.
Science (New York, N.Y.), 230(4731), 1282-1285 (1985-12-13)
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human
Stephanie S Watowich et al.
Immunological reviews, 238(1), 76-92 (2010-10-26)
Understanding the diversification of dendritic cell (DC) lineages is one of the last frontiers in mapping the developmental hierarchy of the hematopoietic system. DCs are a vital link between the innate and adaptive immune responses; thus, elucidating their developmental pathways
Julie M Vose et al.
Leukemia & lymphoma, 50(9), 1412-1421 (2009-07-16)
In autologous stem cell transplantation, mobilized peripheral blood has replaced the bone marrow as the preferred source of hematopoietic stem cells (HSCs). Because HSCs normally exist in the blood in very low numbers, the use of agents to "mobilize" HSCs
Kazuhiro Maki et al.
International journal of hematology, 100(4), 361-369 (2014-07-25)
Myelodysplastic syndrome (MDS) is a stem cell tumor characterized by dysplastic features and ineffective hematopoiesis in the early phase and leukemic progression in the late phase. Speculating that differences in the expression of genes and microRNA (miRNA) in control and
Antonella Mancusi et al.
Blood, 125(20), 3173-3182 (2015-03-15)
Because activating killer cell immunoglobulinlike receptors (KIRs) are heterogeneously expressed in the population, we investigated the role of donor activating KIRs in haploidentical hematopoietic transplants for acute leukemia. Transplants were grouped according to presence vs absence of KIR-ligand mismatches in

Artículos

Read article on hematopoietic cytokines and hematopoiesis

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