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Merck

G0407

Sigma-Aldrich

Granulocyte colony-stimulating factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

product name

Granulocyte colony-stimulating factor human, G-CSF, recombinant, expressed in E. coli, suitable for cell culture

origen biológico

human

Nivel de calidad

recombinante

expressed in E. coli

Análisis

≥95% (SDS-PAGE)

formulario

lyophilized powder

potencia

0.01-0.1 ng/mL

calidad

endotoxin tested

mol peso

18.8 kDa (175 amino acids including N-terminal methionine)

envase

pkg of 5 and 25 μg

técnicas

cell culture | mammalian: suitable

impurezas

<1 EU/μg (LAL test)

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CSF3(1440)

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Descripción general

Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.

Aplicación

Granulocyte colony-stimulating factor human has been used:
  • in culturing human neutrophils
  • as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells
  • to test its protective effects chronic liver damage model

Acciones bioquímicas o fisiológicas

Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Forma física

Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF

Nota de análisis

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Rui Zhang et al.
Hematology (Amsterdam, Netherlands), 20(10), 555-560 (2015-06-13)
The treatment for acute myeloid leukemia (AML) remains an important clinical problem. Recently, hematopoietic stem cell therapy provides promising outcomes. In this study we examined the clinical effect of HLA-mismatched hematopoietic stem cell infusion combined with chemotherapy as a postremission
Antiopi Varelias et al.
Blood, 125(15), 2435-2444 (2015-02-13)
Idiopathic pneumonia syndrome (IPS) is a relatively common, frequently fatal clinical entity, characterized by noninfectious acute lung inflammation following allogeneic stem cell transplantation (SCT), the mechanisms of which are unclear. In this study, we demonstrate that immune suppression with cyclosporin
Akiko Sugiyama et al.
Chronobiology international, 32(7), 934-941 (2015-07-15)
Circulating hematopoietic stem cells exhibit robust circadian fluctuations, which influence the mobilized cell yield, even during enforced stem cell mobilization. However, alterations in the expression of circadian clock genes during granulocyte colony-stimulating factor (G-CSF)-induced peripheral blood stem cell (PBSC) mobilization
Jessy Arbez et al.
Cytotherapy, 17(7), 948-955 (2015-03-31)
This study aimed to characterize the immune effectors contained in the grafts from donor mice mobilized by granulocyte colony-stimulating factor (G-CSF) and plerixafor and to evaluate their impact on the development of acute graft-versus-host-disease (aGVHD). Mobilization was done with G-CSF
Saurabh Agarwal et al.
Cancer research, 75(12), 2566-2579 (2015-04-25)
Increasing evidence suggests that inflammatory cytokines play a critical role in tumor initiation and progression. A cancer stem cell (CSC)-like subpopulation in neuroblastoma is known to be marked by expression of the G-CSF receptor (G-CSFR). Here, we report on the

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