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GE17-5087-02

Sigma-Aldrich

HiPrep™ 26/10 Desalting

Cytiva 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Sinónimos:

Desalting Column, HiPrep Desalting Column

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

Materiales

polypropylene (PP)

mol peso

1000-5000 Da (globular proteins)

envase

pack of 4

fabricante / nombre comercial

Cytiva 17-5087-02

condiciones de almacenamiento

dry media

Parámetros

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

lecho volumen

53 mL

columna L × D.I.

10 cm × 26 mm

Matriz

cross-linked dextran
polymeric

grupo activo de la matriz

dextran-epichlorohydrin phase

tamaño de partícula

17-132 μm (dry)

cleaning

2-13

Intervalo de trabajo

2-13

técnica de separación

size exclusion (SEC)

Descripción general

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Características y beneficios

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Idoneidad

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Información legal

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

IgG Antibody Purification with HiTrap® Protein G HP Columns

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Protocolos

Affinity Chromatography in a Purification Strategy (CIPP)

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Tag Removal by Enzymatic Cleavage

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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