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Key Documents

GE28-9893-36

Sigma-Aldrich

HiLoad® 26/600 Superdex® 200 pg

Cytiva 28-9893-36, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

Sinónimos:

HiLoad Superdex 200

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

Materiales

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

mol peso

10000-600000 Da (globular proteins)

envase

pkg of 1 ea

fabricante / nombre comercial

Cytiva 28-9893-36

Parámetros

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa

lecho volumen

320 mL

columna L × D.I.

60 cm × 26 mm

matriz

agarose-dextran composite
 dextran-agarose particle platform
polymeric

grupo activo de la matriz

dextran-agarose phase
dextran-agarose phase

tam. prom. pieza

34 μm

cleaning

1-14

working range

3-12

técnica de separación

size exclusion (SEC)

Descripción general

HiLoad® 26/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice highly suitable for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Características y beneficios

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Idoneidad

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Información legal

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocolos

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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