Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

GE28-9893-35

Sigma-Aldrich

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Sinónimos:

16/600 Superdex 200, HiLoad Superdex 200

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

Materiales

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

mol peso

10000-600000 Da (globular proteins)

envase

pkg of 1 ea

fabricante / nombre comercial

Cytiva 28-9893-35

Parámetros

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

lecho volumen

120 mL

columna L × D.I.

60 cm × 16 mm

matriz

agarose dextran composite
 dextran-agarose particle platform
polymeric

grupo activo de la matriz

dextran-agarose phase

tam. prom. pieza

34 μm

cleaning

1-14

working range

3-12

técnica de separación

size exclusion (SEC)

Descripción general

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Características y beneficios

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Idoneidad

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Productos recomendados

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Información legal

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Artículos

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Optimizing Purification of Histidine-Tagged Proteins

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

Protocolos

Purification of Mouse Monoclonal IgG1 using HiTrap Protein

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

Affinity Chromatography in a Purification Strategy (CIPP)

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico