CRISPR20

Lenti CRISPR Universal Non-Target Control#3 Plasmid DNA (LV04 vector)

• Código UNSPSC: 41106610
• NACRES: NA.51

Propiedades

• Formulario: liquid
• Nivel de calidad: 200
• envase: vial of 50 μL
• concentración: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA )
• aplicaciones: CRISPR
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The Lenti CRISPR Non-Target Control#3 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Aplicación

Functional Genomics/Screening/Target Validation

Otras notas

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

Información legal

CRISPR Use License Agreement

Lentiviral License Agreement

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable