H0887

HEPES solution

1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent

• Sinónimos: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Número de CAS: 7365-45-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161704
• ID de la sustancia en PubChem: 24895414
• NACRES: NA.75

Propiedades

• Nombre del producto: HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
• esterilidad: sterile-filtered
• Nivel de calidad: 300
• Línea del producto: BioReagent; BioXtra
• Ensayo: 0.9—1.1 M
• Formulario: liquid
• condiciones de almacenamiento: (Tightly closed. Dry); dry at room temperature
• concentración: 1 M
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• color: colorless
• pH: 7.0-7.6
• intervalo de pH útil: 6.8-8.2
• densidad: 1.1 g/cm³
• idoneidad: suitable for component for culture media; suitable for electrophoresis
• aplicaciones: cell analysis; life science and biopharma; sample preparation
• temp. de almacenamiento: 2-8°C
• cadena SMILES: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descripción

Descripción general

HEPES Buffer Solution, a sterile-filtered 1M solution of 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a vital biological buffer in cell biology, biochemical, and biological research. This zwitterionic buffer enjoys widespread adoption due to its effective pH range of 6.8 to 8.2, making it suitable for a diverse array of research applications. HEPES excels as a buffering agent in cell culture media, offering several advantages over alternatives like Tris and phosphate. Notably, HEPES exhibits lower cell toxicity, ensuring optimal cell health and viability during experiments.

Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.

It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used as a component of:

• Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices
• Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity
• cell culture media to provide buffer capacity to maintain the pH of the cell line
• buffer in decellularization of porcine nucleus pulposus

Características y beneficios

• High purity product for biochemical and biological research
• Sterile filtered and cell culture tested
• Ready Solution reduces the need
• Useful for the pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
• Ready available solution reduce the need for preparation time

Precaución

Concentrations of HEPES over 40mM may be toxic to some cell types.

Nota de preparación

Prepared from HEPES free acid and cell culture grade water.

Otras notas

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Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable