H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

• Sinónimos: Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)
• Fórmula empírica (notación de Hill): C₈H₁₈N₂O₄S
• Número de CAS: 7365-45-9
• Peso molecular: 238.30
• Beilstein: 883043
• Número CE: 230-907-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161704
• ID de la sustancia en PubChem: 24895731
• NACRES: NA.75

Propiedades

• Línea del producto: BioXtra
• Análisis: ≥99.5% (titration)
• formulario: powder
• técnicas: cell culture | embryo: suitable
• pH: 5.0-6.5 (25 °C, 238 g/L)
• intervalo de pH útil: 6.8-8.2
• pKa (25 °C): 7.5; 7.5
• solubilidad: water: 500 mg/mL, clear, colorless
• absorción: ≤0.05 at 290 in H₂O at 33%
• cadena SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descripción

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Aplicación

HEPES has been used:

• As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
• As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
• in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Otras notas

This product has been qualified for use in cell and mouse embryo culture.

Easily compare specifications for HEPES products with the HEPES specification table.

Propiedades físicas

Useful pH range: 6.8 - 8.2

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)