H7006
HEPES sodium salt
≥99.0% (titration)
Sinónimos:
Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Fórmula empírica (notación de Hill):
C8H17N2NaO4S
Número de CAS:
Peso molecular:
260.29
Número CE:
Número MDL:
Código UNSPSC:
12161700
ID de la sustancia en PubChem:
NACRES:
NA.25
Productos recomendados
Nivel de calidad
Ensayo
≥99.0% (titration)
Formulario
powder
condiciones de almacenamiento
dry at room temperature
técnicas
HPLC: suitable
color
white
pH
10-12 (1 M in water)
intervalo de pH útil
6.8-8.2
pKa (25 °C)
7.5
solubilidad
water: 0.1 M, clear, colorless
idoneidad
suitable for mass spectrometry
aplicaciones
diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics
temp. de almacenamiento
room temp
cadena SMILES
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
Clave InChI
RDZTWEVXRGYCFV-UHFFFAOYSA-M
¿Está buscando productos similares? Visita Guía de comparación de productos
Categorías relacionadas
Descripción general
HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Aplicación
HEPES buffer finds application in:
It has been used:
- Most studies with metal ions
- Substitute for Tris and phosphate in studies with metal ions
- Grinding buffer in plant studies
- Running buffer in gel electrophoresis
- Buffer in electroporation studies
- Bradford or bicinchoninic acid (BCA) assays
- Extraction buffer to prevent damage of proteins in red blood cells
It has been used:
- as a buffer component in the preparation of a low sucrose buffer
- as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
Características y beneficios
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water
- Negligible metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
Forma física
Lyophilized, spray dried, or crystallized material.
Otras notas
For additional information on our range of Biochemicals, please complete this form.
Producto relacionado
Referencia del producto
Descripción
Precios
producto comparable
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with
Junbo Li et al.
Dalton transactions (Cambridge, England : 2003), 41(13), 3623-3626 (2012-02-24)
We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico