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Merck

H3662

Sigma-Aldrich

HEPES sodium salt solution

1M, BioReagent, suitable for cell culture

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About This Item

Fórmula empírica (notación de Hill):
C8H17N2NaO4S
Número de CAS:
Peso molecular:
260.29
Número MDL:
Código UNSPSC:
12161700
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

esterilidad

sterile-filtered (0.2 μm filtered)

Línea del producto

BioReagent

Formulario

solution

concentración

0.90-1.10 M

técnicas

cell culture | mammalian: suitable

impurezas

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

trazas de catión

Fe: <5 ppm

cadena SMILES

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

Clave InChI

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Descripción general

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Aplicación

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with

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