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Merck

SHC016

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

Sinónimos:

MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

Número MDL:
Código UNSPSC:
41106609
NACRES:
NA.51

Nivel de calidad

Línea del producto

MISSION®

concentración

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Descripción general

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Aplicación

MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
  • in tumor cells for multicolour imaging
  • in human adult low calcium temperature keratinocytes
  • in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)
  • to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation
To see more application data, protocols, vector maps visit sigma.com/shrna.

Información legal

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Zac1 regulates astroglial differentiation of neural stem cells through Socs3
Schmidt EU, et al.
Stem Cells, 31(8), 1621-1632 (2013)
Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes
Avitabile D, et al.
The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)
Brain tumour cells interconnect to a functional and resistant network
Osswald M, et al.
Nature, 528(7580), 93-93 (2015)
Ryusuke Nakajima et al.
PloS one, 12(6), e0179585-e0179585 (2017-06-29)
A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA that contains a SINE (short interspersed element)-B1F motif that was
Sophie Weil et al.
Neuro-oncology, 19(10), 1316-1326 (2017-04-19)
Primary and adaptive resistance against chemo- and radiotherapy and local recurrence after surgery limit the benefits from these standard treatments in glioma patients. Recently we found that glioma cells can extend ultra-long membrane protrusions, "tumor microtubes" (TMs), for brain invasion

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