SHC216

MISSION^® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

• Sinónimos: MISSION^® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
• Número MDL: MFCD07785395
• Código UNSPSC: 41106609
• NACRES: NA.51

Propiedades

• Línea del producto: MISSION^®
• concentración: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The MISSION^® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION^® shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Aplicación

To see more application data, protocols, vector maps visit sigma.com/shrna.

Información legal

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable