C9282

Colato de sodio hydrate

BioReagent, suitable for cell culture

• Sinónimos: Ácido 3α,7α, 12α-trihidroxi-5β-colan-24-oico sodium salt, Ácido colálico sodium salt
• Fórmula empírica (notación de Hill): C₂₄H₃₉NaO₅ · xH₂O
• Número de CAS: 206986-87-0
• Peso molecular: 430.55 (anhydrous basis)
• Beilstein: 3582354
• Número CE: 206-643-5
• Número MDL: MFCD00150749
• Código UNSPSC: 12161902
• ID de la sustancia en PubChem: 24893147
• NACRES: NA.75

Propiedades

• origen biológico: bovine bile; ovine bile
• Nivel de calidad: 200
• descripción: anionic
• Línea del producto: BioReagent
• Ensayo: ≥99.0% (HPLC)
• Formulario: powder
• mol peso: micellar avg mol wt 900-1300
• número de agregación: 2-3
• técnicas: cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• solubilidad: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• cadena SMILES: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1
• Clave InChI: MUVVIYFKOVLQHL-RCVKHMDESA-M

Descripción

Descripción general

Cholate is a primary bile acid produced from cholesterol in the liver. It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).

Aplicación

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system,
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor

Acciones bioquímicas o fisiológicas

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.

Información de seguridad

• Frases de peligro: H412
• Consejos de prudencia: P273 - P501
• Clasificaciones de peligro: Aquatic Chronic 3
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)