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Merck

A7437

Sigma-Aldrich

Acetate Kinase from Escherichia coli

lyophilized powder, ≥150 units/mg protein (biuret)

Sinónimos:

ATP:Acetate phosphotransferase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Formulario

lyophilized powder

Nivel de calidad

actividad específica

≥150 units/mg protein (biuret)

composición

Protein, 5.0-20.0%

Nº de acceso UniProt

actividad extraña

adenosine triphosphatase and NADH oxidase ≤0.01%

temp. de almacenamiento

−20°C

Información sobre el gen

Escherichia coli K12 ... ackA(946775)

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Descripción general

Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.

Aplicación

Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .

Acciones bioquímicas o fisiológicas

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.
Involved in the metabolism of propanoate, pyruvate and taurine

Definición de unidad

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

Forma física

Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281
Kornmann H, et al.
Applied Microbiology and Biotechnology, 62(2-3), 168-173 (2003)
Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
Buss KA, et al.
Journal of Bacteriology, 183(2), 680-686 (2001)
S C Olson et al.
The Journal of biological chemistry, 266(26), 17236-17242 (1991-09-15)
Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in
F J Grundy et al.
Journal of bacteriology, 175(22), 7348-7355 (1993-11-01)
The Bacillus subtilis gene encoding acetate kinase was identified on the basis of sequence similarity to the Escherichia coli ackA gene and to a second E. coli gene closely related to ackA. Insertional inactivation of this region of the B.
Hélène Scornec et al.
Frontiers in microbiology, 11, 156-156 (2020-03-21)
The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the

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