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Merck

10128023001

Roche

NADH

Grade II, disodium salt

Sinónimos:

β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH

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About This Item

Fórmula empírica (notación de Hill):
C21H27N7Na2O14P2 · xH2O
Número de CAS:
Peso molecular:
709.40 (anhydrous basis)
Número MDL:
Código UNSPSC:
12352204
ID de la sustancia en PubChem:

Nivel de calidad

Análisis

98%

formulario

amorphous powder

mol peso

(NADH-Na2: Mr = 709.4
NADH: Mr = 665.4)

envase

pkg of 1 g

fabricante / nombre comercial

Roche

condiciones de almacenamiento

protect from light

λmáx.

260 and 340 nm

Condiciones de envío

wet ice

cadena SMILES

O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O

InChI

1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1

Clave InChI

FWBNCIFELNNJCX-UHOVGGJYSA-L

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Aplicación

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.
NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.

Acciones bioquímicas o fisiológicas

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Envase

Packaged by solid weight.

Calidad

Purity: 98% β-NADH-Na2 based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A260 nm-A340 nm: <2.40
Organic solvents: <4% (GC)

Especificaciones

Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at 334 nm (Hg), 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at 365 nm (Hg)

Formula:
C21H27N7O14P2Na2

pH value in water: 7.5 ± 0.5 (100 mg/ml)

Forma física

Amorphous powder

Nota de preparación

Activator: Mg2+ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C

Almacenamiento y estabilidad

Store at 2 to 8 °C. (Store dry!)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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