A2103

Anti-Actin, N-terminal antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Actin Detection Antibody, Rabbit Anti-Actin
• Número MDL: MFCD00145889
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 42 kDa
• reactividad de especies: frog, rat, mouse, chicken, human
• envase: antibody small pack of 25 μL
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• concentración: ~0.5 mg/mL
• técnicas: immunocytochemistry: 1-2 μg/mL using cultured chicken fibroblasts; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using whole extract of the human epitheloid carcinoma HeLa cell line.; western blot: 2-4 ng/mL using whole extract of rat skeletal muscle
• Nº de acceso UniProt: P63261
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ACTA1(58) , ACTA2(59) , ACTB(60) , ACTC1(70) , ACTG1(71) , ACTG2(72) ; mouse ... Acta1(11459) , Acta2(11475) , Actb(11461) , Actc1(11464) , Actg1(11465) , Actg2(11468) ; rat ... Acta1(29437) , Acta2(81633) , Actb(81822) , Actc1(29275) , Actg1(287876) , Actg2(25365)

Descripción

Descripción general

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Actin is one of the most conserved eukaryotic proteins, which is expressed in mammals and birds as at least six major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported.

Inmunógeno

synthetic actin N-terminal nonapeptide conjugated to KLH.

Aplicación

Anti-Actin, N-terminal antibody produced in rabbit has been used in

• immunoblotting
• quantitative chromatin immunoprecipitation (qChIP)

Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.

Rabbit anti-actin, N-terminal antibody can be used for western blotting assays. The antibody can also be used for immunocytochemistry at 1-2μg/mL using cultured chicken fibroblasts. Furthermore, the antibody is suitable for use in immunohistochemistry at a concentration of 2-4μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle.

Acciones bioquímicas o fisiológicas

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coexist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Otras notas

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)