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F3046

Sigma-Aldrich

Monoclonal Anti-Actin–FITC antibody produced in mouse

clone AC-40, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Monoclonal Anti-Actin

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

conjugado

FITC conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

AC-40, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 42 kDa

reactividad de especies

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

técnicas

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

isotipo

IgG2a

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ACTA1(58) , ACTA1(58) , ACTA1(58) , ACTA2(59) , ACTA2(59) , ACTA2(59) , ACTB(60) , ACTB(60) , ACTB(60) , ACTC1(70) , ACTC1(70) , ACTC1(70) , ACTG1(71) , ACTG1(71) , ACTG1(71) , ACTG2(72) , ACTG2(72) , ACTG2(72)
mouse ... Acta1(11459) , Acta1(11459) , Acta1(11459) , Acta2(11475) , Acta2(11475) , Acta2(11475) , Actb(11461) , Actb(11461) , Actb(11461) , Actc1(11464) , Actc1(11464) , Actc1(11464) , Actg1(11465) , Actg1(11465) , Actg1(11465) , Actg2(11468) , Actg2(11468) , Actg2(11468)
rat ... Acta1(29437) , Acta1(29437) , Acta1(29437) , Acta2(81633) , Acta2(81633) , Acta2(81633) , Actb(81822) , Actb(81822) , Actb(81822) , Actc1(29275) , Actc1(29275) , Actc1(29275) , Actg1(287876) , Actg1(287876) , Actg1(287876) , Actg2(25365) , Actg2(25365) , Actg2(25365)

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Descripción general

Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.

Inmunógeno

synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone

Aplicación

Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.
Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Acciones bioquímicas o fisiológicas

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Otras notas

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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K Sutoh et al.
Biochemistry, 25(20), 6186-6192 (1986-10-07)
An antibody was raised against the N-terminal 18 residues of rabbit skeletal muscle actin. By the use of this antibody as the N-terminal probe of actin and the fluorescent label at Cys-374 as its C-terminal probe, binding sites of depactin
J L Lessard
Cell motility and the cytoskeleton, 10(3), 349-362 (1988-01-01)
Two IgG1, kappa monoclonal antibodies (Mab) against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen. One Mab, designated B4, shows a preferential reactivity toward enteric smooth muscle actin but also cross-reacts
Kimberly A Moore et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 232(2), 268-281 (2004-12-23)
Local alterations in the mechanical compliance of the basement membrane that alter the level of isometric tension in the cell have been postulated to influence tissue morphogenesis. To explore whether cell tension contributes to tissue pattern formation in vivo, we
I M Herman
Current opinion in cell biology, 5(1), 48-55 (1993-02-01)
The actin supergene family encodes a number of structurally related, but perhaps functionally distinct, protein isoforms that regulate contractile potential in muscle tissues and help to control the shape as well as the motility of non-muscle cells. In spite of
The actin gene family: function follows isoform.
Perrin B J and Ervasti J M
Cytoskeleton (Hoboken, N.J.), 67(10), 630-634 (2010)
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