A2172

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse

clone 5C5, ascites fluid

• Sinónimos: Mouse Anti-Actin
• Número MDL: MFCD00164521
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: 5C5, monoclonal
• contiene: 15 mM sodium azide
• reactividad de especies: rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle; immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle; indirect ELISA: suitable; western blot: suitable
• isotipo: IgM
• Nº de acceso UniProt: P68032; P68133
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ACTA1(58) , ACTC1(70) ; mouse ... Rwdd2a(69519) ; rat ... Acta1(29437) , Actc1(29275)

Descripción

Descripción general

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Inmunógeno

rabbit striated muscle actin.

Aplicación

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used

• immunofluorescence
• double immunostaining
• double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Acciones bioquímicas o fisiológicas

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Forma física

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Otras notas

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable