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KAPA Taq EXtra HotStart ReadyMix

suitable for PCR

• Sinónimos: KAPA, PCR, hotstart PCR, taq DNA polymerase
• Código UNSPSC: 41106300
• NACRES: NA.55

Propiedades

• Características: dNTPs included; hotstart
• Nivel de calidad: 100
• envase: pkg of 1.25 mL (KK3604); pkg of 6.25 mL (KK3605)
• fabricante / nombre comercial: Roche
• técnicas: PCR: suitable
• entrada: purified DNA
• temp. de almacenamiento: −20°C

Descripción

Descripción general

KAPA Taq EXtra HotStart^® ReadyMix with dye (2X) is a ready-to-use cocktail containing all components for short- to long-range PCR, except primers and template. The 2X ReadyMix contains KAPA Taq EXtra HotStart DNA Polymerase (0.675 U per 25 μL reaction), KAPA Taq EXtra Buffer (1X), dNTPs (0.3 mM of each dNTP at 1X), MgCl₂ (2 mM at 1X) and stabilizers. The 2X ReadyMix also contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.

Aplicación

KAPA Taq EXtra HotStart^® ReadyMix^™ has been used for:

• PCR of amplicons to be analyzed by agarose gel electrophoresis
• PCR of short and medium length targets (<5 kb)
• Long-range PCR (5 – 15 kb)
• PCR with limiting amounts of template DNA

Características y beneficios

• KAPA Taq EXtra HotStart^® is a blend of KAPA Taq HotStart and a proofreading DNA polymerase
• Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA
• Fidelity is 2–4 times better than Taq
• For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
• Amplify mid- to long-range targets with high yield and sensitivity.
• 2X ReadyMix with dye contains two inert tracking dyes to allow loading of PCR products directly onto agarose gels for analysis.

Calidad

Each batch of KAPA Taq EXtra HotStart^® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The 2X KAPA Taq EXtra HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Nota de preparación

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Otras notas

For Research Use Only. Not for use in diagnostic procedures.

Información legal

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Solo componentes del kit

• KAPA Taq EXtra HotStart^® DNA Polymerase 0.675U per 25μL reaction
• KAPA Taq EXtra Buffer (1X)
• 1X dNTPs 0.3 mM of each dNTP
• 1X MgCl₂ 2 mM
• Stabilizers
• Two inert tracking dyes

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash