ECM103
Human Laminin
MILLICOAT® Human Laminin Coated Strips (96-Wells), suitable for cell culture
Sinónimos:
Formerly under the CytoMatrix brand name.
About This Item
Productos recomendados
product name
Millicoat® Human Laminin Coated Strips (96-Wells), 96-well plate coated with human laminin used for cell adhesion studies.
Nivel de calidad
reactividad de especies
human
fabricante / nombre comercial
Chemicon®
Millicoat®
técnicas
activity assay: suitable
cell based assay: suitable
entrada
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
Nº de acceso NCBI
Nº de acceso UniProt
método de detección
colorimetric
Condiciones de envío
wet ice
Descripción general
Aplicación
NOTE: Optimal assay timing and performance may vary for different cell lines but generally can be obtained using subconfluent cell cultures in the assay described below. Subconfluent cultures can be achieved by splitting cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydrated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 2-3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO4, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.
6.Determine the absorbance at 540 - 570 nm on a microplate reader.
Cell Structure
Envase
Almacenamiento y estabilidad
Información legal
Cláusula de descargo de responsabilidad
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
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Artículos
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocolos
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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