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P5899

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Sinónimos:

PDL HBr

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About This Item

Número de CAS:
25988-63-0
Número MDL:
MFCD00237230
Código UNSPSC:
12352202
eCl@ss:
32160406
ID de la sustancia en PubChem:
24898718
NACRES:
NA.75

product name

Poli-L-lisina hydrobromide, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

origen biológico

synthetic (organic)

Nivel de calidad

200

esterilidad

γ-irradiated

Línea del producto

BioXtra

formulario

lyophilized powder

mol peso

≥300,000

envase

pkg of 5 mg

técnicas

cell culture | mammalian: suitable

cobertura de la superficie

4 μg/cm2

impurezas

<10% water (by Karl Fischer)

solubilidad

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

cadena SMILES

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

Clave InChI

WBSCNDJQPKSPII-KKUMJFAQSA-N

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Aplicación

Poly-L-lysine hydrobromide has been used to coat:
  • 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation
  • flat bottom 96-well plates for in vitro neuronal stem cell differentiation
  • T75 flasks for primary astrocytic cultures

Acciones bioquímicas o fisiológicas

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Componentes

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Precaución

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Nota de análisis

Molecular weight based on viscosity. Also assayed by MALLS.

Otras notas

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Malgorzata J Podolska et al.
Journal of leukocyte biology, 106(6), 1359-1366 (2019-09-04)
The release of neutrophil extracellular traps (NETs) is one of the weapons neutrophils have in their armory. NETs consist of extracellular chromatin fibers decorated with a plethora of cytoplasmic and granular proteins, such as the antimicrobial serine protease neutrophil elastase
Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Skye E Smith et al.
eNeuro, 7(4) (2020-08-02)
Aberrant migration of inhibitory interneurons can alter the formation of cortical circuitry and lead to severe neurologic disorders including epilepsy, autism, and schizophrenia. However, mechanisms involved in directing the migration of interneurons remain incompletely understood. Using a mouse model, we
Stuart B Mazzone et al.
Molecular neurobiology, 57(2), 949-963 (2019-10-21)
Bronchopulmonary sensory neurons are derived from the vagal sensory ganglia and are essential for monitoring the physical and chemical environment of the airways and lungs. Subtypes are heterogenous in their responsiveness to stimuli, phenotype, and developmental origin, but they collectively
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