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10838039001

Roche

Fibronectina

from human plasma

Sinónimos:

Fibronectin

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About This Item

Número MDL:
MFCD00131062
Código UNSPSC:
12352207

origen biológico

human plasma

Nivel de calidad

100

esterilidad

non-sterile; 0.2 μm filtered

Análisis

95% (SDS-PAGE)

formulario

lyophilized (clear, colorless solution after reconstitution)

mol peso

440 kDa

envase

pkg of 5 mg

fabricante / nombre comercial

Roche

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

técnicas

cell culture | mammalian: suitable

Nº de acceso UniProt

P02751

temp. de almacenamiento

2-8°C

Información sobre el gen

human ... FN1(2335)

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Descripción general

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Especificidad

Active on most mammalian cells

Aplicación

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.

Método de análisis

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Forma física

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Nota de preparación

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitución

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolos

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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