ECM205
Millicoat® ECM Screening Kit, 1 ea. ECM101-ECM105
Millicoat®, pkg of 96-well plate(s) (for fibronectin, vitronectin, laminin, collagen I & collagen IV)
Sinónimos:
Formerly under the CytoMatrix brand name.
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352202
eCl@ss:
32161000
NACRES:
NA.77
Productos recomendados
reactividad de especies
human
Nivel de calidad
fabricante / nombre comercial
Chemicon®
Millicoat®
envase
pkg of 96-well plate(s) (for fibronectin, vitronectin, laminin, collagen I & collagen IV)
técnicas
activity assay: suitable
cell based assay: suitable
entrada
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
método de detección
colorimetric
Condiciones de envío
wet ice
Descripción general
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat® Cell Adhesion Strips are provided as 12 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with a human ECM protein. Row H of each strip is coated with BSA which serves as a negative assay control. The Millicoat screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I and collagen IV. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
Aplicación
PROCEDURE:
NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.
6. Determine the absorbances at 540 - 570 nm on a microplate reader.
NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.
6. Determine the absorbances at 540 - 570 nm on a microplate reader.
Research Category
Cell Structure
Cell Structure
The Millicoat® screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV.
Almacenamiento y estabilidad
May be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLICOAT is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
11 - Combustible Solids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Tomonori Kaneda et al.
Cancer letters, 270(2), 354-361 (2008-07-09)
This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of
K E Crawford et al.
Placenta, 33(5), 416-423 (2012-03-02)
Calreticulin is a calcium binding, endoplasmic reticulum resident protein best known for its roles in intracellular calcium homeostasis and the quality control processes of the endoplasmic reticulum. There is evidence for a range of activities for calreticulin outside the endoplasmic
Rosalyn D Blumenthal et al.
Cancer research, 65(19), 8809-8817 (2005-10-06)
CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. The effects of three monoclonal antibodies targeting different epitopes on these antigens (NH2-terminal [MN-3] and A1B1 domains [MN-15] shared by CEACAM5 and CEACAM6 and the
Guangchun Sun et al.
Journal of Cancer, 6(10), 996-1004 (2015-09-15)
Hepatocellular carcinoma (HCC) is a type of malignant cancer. Notch signaling is aberrantly expressed in HCC tissues with more evidence showing that this signaling plays a critical role in HCCs. In the present study, we investigate the effects of the
Mary Ann Stepp et al.
Journal of cell science, 120(Pt 16), 2851-2863 (2007-08-02)
We have reported previously that syndecan-1 (Sdc1)-null mice show delayed re-epithelialization after skin and corneal wounding. Here, we show that primary keratinocytes obtained from Sdc1-null mice and grown for 3-5 days in culture are more proliferative, more adherent and migrate
Artículos
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocolos
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico